A tech-geek, Donatello, always gets excited when seeing new technologies. He is responsible for making many of the ninja gadget and vehicles of the Turtles. He is not only a genius mechanic, but also an expert in using the bo staff, his signature weapon. Donatello's chapter in the GBA game revolves around saving Raphael from the Foot Tech Ninjas who have captured him.
He also appears in Teenage Mutant Ninja Turtles (2003 video game), Teenage Mutant Ninja Turtles 2: Battle Nexus, TMNT: Mutant Melee, and Teenage Mutant Ninja Turtles 3: Mutant Nightmare.