- Leonardo (Cam Clarke)
- Donatello (Barry Gordon)
- Michelangelo (Townsend Coleman)
- Raphael (Rob Paulsen)
- Hamato Yoshi/Splinter (Peter Renaday)
- April O'Neil (Renae Jacobs)
- Irma Langinstein (Jennifer Darling)
- Shredder (James Avery)
- Krang (Pat Fraley)
- Rocksteady (Cam Clarke)
- Bebop (Barry Gordon)
- Garson Grunge/Muckman (Townsend Coleman)
- Joe Junkee/Joe Eyeball (Rob Paulsen)
- Tropotron Labs security guard (Peter Renaday)
- News anchor (Cam Clarke)
Home media releases
- Teenage Mutant Ninja Turtles Season 5
- Teenage Mutant Ninja Turtles: The Complete Classic Series Collection
- A small flap which looks to be the Transport Module's door and it's seat appear on the side of the Module nearest the drill when Shredder and the mutants flee the lab after the Turtles intercept them.
- The Mutagen vat is seen as full after Splinter tipped out it's contents out on Krang and Shredder earlier.
- When Muckman said that the Turtles are a real menace, he spoke with Joe Eyeball's voice.
- When Shredder and the mutants arrived back at the city to locate Muckman and Joe Eyeball, the module door opened downwards instead of up and was missing it's window.
- Krang's corneas are coloured dark purple for this episode and "Landlord of the Flies".
- Shredder's piece of dialogue is spoken by Jim Cummings when he and the mutants head back to the Module.