The new 2D series is co-executive produced by Andy Suriano (character designer for Samurai Jack) and Ant Ward (supervising producer of the 2012 TV series). The series is said to be lighter and more humor-oriented than the 2012 series.
- April O'Neil (Kat Graham)
- Donatello (Josh Brener)
- Leonardo (Ben Schwartz)
- Michelangelo (Brandon Mychal Smith)
- Raphael (Omar Benson Miller)
- Splinter (Eric Bauza)
- This is the first time April is of a different ethnicity than previous incarnations as she is African-American instead of Irish-American.
- Raphael is the leader of the TMNT for the first time instead of Leonardo.