The Sopho University is a university that appears in the episode Donatello's Degree of the 1987-1996 animated series and briefly in Too Hot to Handle. It provides by-mail college degrees, one of which was earned by Donatello.

The university has its own railway station, and it borders the Bottomless Lake. Donatello and Irma traveled there by train to meet Professor Filo Sopho,[1] and stayed in the university's campus for a graduation ceremony. However, Donatello soon discovered that Sopho University was also the staging ground for Sopho's plan to spin everything off the face of the Earth.

After Sopho's arrest, the university was abandoned and fell into disrepair.