The goal of the game is to complete a number of missions, consisting of side-scrolling levels, culminating in a final battle with the Shredder.
The game is mainly based on the continuity of the original Mirage comics(the opening cutscene even uses some lines from the first issue regarding the Turtles' battle with the Purple Dragons and Splinter telling the Turtles their origin). The game also has characters and elements from the cartoon(multi-colored bandannas, Baxter Stockman appearing as a Caucasian blond, Bebop and Rocksteady included as bosses, the Turtles' taste for pizza, and April O'Neil being a news reporter and wearing a yellow jumpsuit) and the film series(the inclusion of Tatsu as a boss, and April O'Neil resembles Paige Turco, who played her Teenage Mutant Ninja Turtles II: The Secret of the Ooze).
Despite being based on the Mirage Comics version, the game is still kid-oriented(e.g., the Shredder is turned over to the police in the ending as opposed to being killed).
The gameplay is loosely similar to the original Prince of Persia. In between missions the Turtles can rest, regaining lost hit points, but the player only has a limited amount of time in which to find the Shredder. The game is designed to be played with a keyboard, and utilizes a key to switch between walking and fighting modes. Each Turtle has the ability to arm and withdraw his weapon. Each Turtle wields his signature weapon and a number of shuriken. Holding the enter key makes the Turtles attack, and the spacebar is used to block enemy attacks based on which arrow key is held.
- Triceraton (fought more than once)
- Foot Ninja
- Labb Ratt (fought more than once)
- Usub Gerstalk